Microbial enzymes represent one of the most dynamic and versatile tools in modern biochemistry and biotechnology. These biomolecules, derived from bacteria, fungi, and actinomycetes, play an essential role in catalyzing biochemical reactions fundamental to life. Their structural diversity, catalytic efficiency, and adaptability have made them indispensable in numerous industrial sectors, including pharmaceuticals, food processing, agriculture, bioenergy, and environmental management. This book, Microbial Enzymes: Biochemical Foundations, Industrial Applications, and Biotechnological Innovations, provides a comprehensive understanding of the biochemical mechanisms, molecular structures, and functional dynamics of microbial enzymes. It integrates fundamental biochemical principles with cutting-edge advances in enzyme engineering, molecular biology, and synthetic biotechnology. The text emphasizes the methods of enzyme discovery, purification, characterization, and genetic modification for enhanced stability and specificity. Additionally, the book explores the industrial potential of microbial enzymes, highlighting their roles in green chemistry, renewable bioresource utilization, and sustainable manufacturing processes. Attention is also given to emerging fields such as metagenomics, protein design, and nanobiocatalysis, which have transformed the scale and efficiency of enzymatic applications. Through a balanced combination of theoretical insight and practical case studies, this volume aims to serve as a foundational and reference text for students, educators, and professionals in biochemistry, microbiology, and biotechnology.